Calcium Imaging

In vivo calcium imaging is a technique utilized to quantify active neuronal populations in response to a stimulus. This system functions through expressing GCaMP that includes an a calmodulin domain attached to EGFP. During active firing of neurons, when intracellular calcium levels increase, calmodulin binds calcium and increases the fluorescence of EGFP, which can be visualized via confocal microscopy.

This video was produced by Jordan Tyrrell, previous lab manager of the Ni Lab.